Well pump liquid endosperm
This application claims priority to provisional application No. The invention relates to nutritional, cosmetic and pharmaceutical compositions containing active ingredients from fresh green coconuts, and methods for obtaining the compositions. Coconut water is the liquid endosperm of Cocos nucifera L an is used as a supplement in media for the growth of plant tissue cultures.
The coconut fruit is unique in that it accumulates large amounts of this liquid over periods of a year or more in its life cycle. The greatest amount of coconut water is found in young, green coconuts and provides nourishment for the growth of the solid endosperm coconut meat inside the hard shell of the fruit.
When the fruit matures, both the well pump liquid endosperm endosperm and the remaining coconut water serve as nutrients for the developing embryo and seedling. Thus coconut water serves as a natural reservoir of nutrients to promote tissue growth 1. The nutritional composition of coconut water obtained from fruits at different stages of maturity has been determined.
The medium is rich in proteins, amino acid sugars, vitamins, minerals and growth hormones Table 1 essential to the promotion of tissue growth. In addition, shikimic acids and quinic acids have been detected in samples of coconut water from fruits at different stages of maturity, with the well pump liquid endosperm amounts being found in young green coconuts. The probable role of these alicyclic acids in romatic biosynthesis, indicates their importance in the developing well pump liquid endosperm.
They may also play a significant role in the nutrition of plant and tissue cultures. The role of RNA in amino acid transport and respiratory metabolism of living cells is well known. The RNA of coconut water would therefore effectively carry out these and other functions as part of the metabolic machinery essential to the developing endosperm tissue of the coconut and therefore support the growth of other living cells as well, in tissue culture media.
Cytokinins are a class of plant growth substances plant hormones active in promoting cell division. They are also involved in cell growth and differentiation and in other physiological processes.
A major cytokinin found in coconut milk was isolated using a standard procedure, the tobacco callus growth-promoting assay. The structure was determined to be a complex trans-zeatin riboside G3A2-ZR.
The nutritive properties of coconut water are well recognized in Oriental tradition. Immature coconuts are used as a glucose-electrolyte oral re-hydration solution for treatment of diarrhea 4. Coconut water solids also provide a nutritive medium for beneficial microflora in the gastrointestinal tract. A recent report also mentions the successful use of coconut water as a short-term intravenous hydration fluid 5. Coconut water has been used traditionally as a nutritional beverage.
The FAO well pump liquid endosperm recently granted a patent in the UK for the manufacture of bottled coconut water that retained all nutrients. This is the first patent given to a UN agency. Unlike existing methods their process did not use HTST pasteurization, wherein heat treatment would inactivate some of the biological activity of the coconut water.
Other researchers have obtained an extract from coconut water and milk called Cocogro, well pump liquid endosperm natural product comprised of plant growth hormones and regulators, well pump liquid endosperm enhances the growth of vegetables, legumes fruit trees, cereal crops, flowering plants, etc. Green coconut milk is reported to be an effective culture medium for animal cells Rev Bras Biol. Although coconut milk has been used traditionally on the skin as a nourishing agent, green coconut water provides unique nutrients for sustaining cell growth.
For example, a protein fraction isolated from coconut water was absent in the milk Birosel, DM et al. Farm Bioquim Univ Sao Paulo Coconut milk is used in a facemask in U. Coconut water was found to be safe for topical use.
Accordingly, coconut water is nontoxic. Coconut water is described as an well pump liquid endosperm sports drink. The comparative properties of coconut water and conventional sports drinks are listed in Table 3 An object of the invention relates to compositions containing as active ingredients, components obtained from coconut water.
The compositions are specifically formulated for use in tissue culture media, more preferably, in supporting or well pump liquid endosperm cells and mammalian tissues derived from ectoderm, mesoderm and endoderm. The composition may be used in topical applications, for example, skin and hair care formulations. The composition may also be formulated into a nutritional supplement so as to be administered by ingestion or intravenously, thereby providing essential nutrients and repair material for the tissues.
The active ingredients of coconut water can be obtained by freeze-drying, and this simple method yields a unique and stable composition with optimum biological well pump liquid endosperm. The inventive composition of the invention differs unexpectedly from that found in well pump liquid endosperm coconut water, and a list of its components is presented in Table 4.
This is based on literature reports wherein the RNA-P of Another object of the invention is a unique process for preserving the natural growth promoting properties of green coconut water. The nutritional properties of green coconut water are optimized by a lyophilization process yielding a microbiologically stable preparation that retains the biological activity of the ingredients. Available as a powder, the material of the invention can be conveniently stored and transported for commercial applications.
The process employs a lyophilization technique, which preserves the biological activity of the well pump liquid endosperm ingredients. The amorphous nature of the freeze dried material protects the protein components during pulverization and storage.
During storage, the material transforms into the more stable crystalline state which is also well pump liquid endosperm hygroscopic. The amorphous material is pulverized under low temperature conditions to yield a powder. The shikimic and quinic acids content and their activity in well pump liquid endosperm dried powder provide unexpected advantages well pump liquid endosperm the fresh material.
The shikimic acid pathway is reported to produce salicylic acid, a known growth promoter in plants. In other systems, shikimic acid and quinic acid well pump liquid endosperm as potent antioxidants. Indoleacetic acid plant well pump liquid endospermthe activity of which is retained in the dried product, has been shown to protect mammalian cells sperm cells.
The product can be used in topical and cosmetic formulations to promote cell growth well pump liquid endosperm as in skin care and hair care formulations as well as in dry blends for nutritional and sports beverages. The invention is also useful as an inhibitor of tumor cell proliferation in mammalian cells. Year of fee payment: A nutritional, cosmetic and pharmaceutical compositions containing active ingredients from the liquid endosperm of fresh green coconuts, and a method for obtaining the active ingredients.
A lyophilized, nutritional composition from liquid endosperm of fresh green coconuts Cocos nucifera L. The composition of claim 1wherein the cytokinins are at well pump liquid endosperm concentration range of 0. The composition of claim 1wherein the indoleacetic acid is at a concentration range of 0. The composition of claim 1wherein the composition is a powder. A cosmetic formulation comprising a composition of claim 1. A nutritional supplement for a beverage comprising a composition of claim 1.
A process for obtaining a biologically active powder from fresh green coconuts Cocos nucifera L. The process of claim 10wherein the lyophilizing of step c occurs at a pressure of from 0. US USB2 en Method of preparation and use of coconut water in mamalian tissue nourishment growth and healthy maintenance. The method of preparation and use of coconut water in mamalian tissue nourishment, growth and healthy maintenance. Extracting active ingredients in mature Coco nucifera water, where the water is taken in a well pump liquid endosperm manner by suction using a trocar connected to a peristaltic pump, introduced in one of germinal centers of the nut previously decontaminated.
Phytoestrogen supplement prepared from pomegranate well pump liquid endosperm and a herbal mixture or coconut milk. English language abstract of CN 1,, Dec. Favorable effects of the antioxidants sodium and magnesium thiazolidine carboxylate on the vitality and life span of Drosophila and mice. Yields, compositions, and nutritive evaluation of barley silages at three stages of maturity for lactating cows.
Nutritive characterization of purslane accessions as influenced by planting date. Changes in chemical composition of the well pump liquid endosperm of red kidney bean plants treated with 2, 4-dichlorophenoxyacetic acid. Formation of flavonoids and chlorogenic acid in apples as affected by crop load. A selenium yeast product, a method of preparing a selenium yeast product and the use of the product for preparing food, a dietary supplement or a drug. Micronutrients and anti-nutritional well pump liquid endosperm of selected tropical vegetables grown in South East, Nigeria.
The Nutritive Value of Some Legumes. Lathyrism in the Rat. Chemical composition of Cola acuminata and Garcinia kola seeds grown in Nigeria. Effect of tannins from faba beans Vicia faba on the digestion of starch by growing chicks.